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Literature summary for 3.1.25.1 extracted from

  • Ishida, M.; Kanamori, Y.; Hori, N.; Inaoka, T.; Ohtsuka, E.
    In vitro and in vivo activities of T4 endonuclease V mutants altered in the C-terminal aromatic region (1990), Biochemistry, 29, 3817-3821.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
DELTAC127 C127-truncated, total loss of activity Tequatrovirus T4
K130L almost same activity as wild-type Tequatrovirus T4
T127M almost same activity as wild-type, Km value in the same order as that of wild-type Tequatrovirus T4
W128A total loss of activity Tequatrovirus T4
W128S only slight activity remains Tequatrovirus T4
Y129A total loss of activity Tequatrovirus T4
Y131A only slight activity remains Tequatrovirus T4
Y132A only slight activity remains Tequatrovirus T4

General Stability

General Stability Organism
addition of 8 M urea leads to denaturation Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000015
-
DNA 14mer containing a TT-dimer WT-enzyme and T127M Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4
-
?
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
T127M Tequatrovirus T4

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate cleavage on the 5'-side of dimers Tequatrovirus T4
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate 2-step mechanism, pyrimidine dimer-DNA glycosylase and an apurinic/apyrimidinic endonuclease Tequatrovirus T4

Renatured (Commentary)

Renatured (Comment) Organism
-
Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Tequatrovirus T4 ?
-
?
DNA 14mer containing a TT-dimer + H2O
-
Tequatrovirus T4 ?
-
?
oligodeoxyribonucleotides 14mer containing a TT-dimer, duplex Tequatrovirus T4 oligonucleotides
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00833
-
DNA 14mer containing a TT-dimer T127M Tequatrovirus T4
0.0183
-
DNA 14mer containing a TT-dimer WT-enzyme Tequatrovirus T4