Cloned (Comment) | Organism |
---|---|
synthetic gene, expressed in Escherichia coli | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000009 | - |
DNA 18mer containing a TT-dimer | - |
Tequatrovirus T4 | |
0.000019 | - |
DNA 14mer containing a TT-dimer | - |
Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
SDS-PAGE | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Tequatrovirus T4 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage near pyrimidine dimers to products with 5'-phosphate | minimum chain length: longer than 10 nucleotides | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
DNA 14mer containing a TT-dimer + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
DNA 18mer containing a TT-dimer + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
oligodeoxyribonucleotides | UV-irradiated, 14mer and 18mer, single-stranded oligomers are a poorer substrate than the corresponding duplexes | Tequatrovirus T4 | oligonucleotides | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0183 | - |
DNA 18mer containing a TT-dimer | - |
Tequatrovirus T4 | |
0.02 | - |
DNA 14mer containing a TT-dimer | - |
Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH-optimum of glycosylase activity | Tequatrovirus T4 |
6.5 | - |
pH-optimum of endonuclease activity | Tequatrovirus T4 |