Literature summary for 3.1.22.1 extracted from

Lepretre, C.; Scovassi, A.I.; Shah, G.M.; Torriglia, A.
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis (2009), Int. J. Biochem. Cell Biol., 41, 1046-1054.

Search for more literature for 3.1.22.1

Application

Application	Comment	Organism
additional information	caspase-independent apoptosis induced by hexamethylene amiloride, LEI/L-DNase II can activate PARP-1 and promote its auto-poly(ADP-ribosyl)ation, thus inhibiting PARP-1 activity. Overexpression of LEI blocks the pro-survival effect of PARP-1 in this model of cell death	Sus scrofa

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21(DE3) pLysS strain transformed with a pET 23d(+) plasmid coding for LEI with a 6x His tag. PZeo-LEI wild-type transfected into HeLa cells, MEF cell lines or BHK-21 cells	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	despite having putative poly(ADP-ribose)-acceptor sites, LEI/L-DNase II is neither significantly poly(ADP-ribosyl)ated nor inhibited by PARP-1 during caspase-independent apoptosis	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by His-select cartridges	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	in vitro physical interaction between LEI/L-DNase II and PARP-1. positive effect of LEI (and, to a lesser extent, of L-DNase II) on PARP-1 activity	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
plasmid DNA + H2O	-	Sus scrofa	?	-	?

Synonyms

Synonyms	Comment	Organism
LEI/L-DNase II	-	Sus scrofa
leukocyte elastase inhibitor-derived DNase II	-	Sus scrofa
leukocyte elastase inhibitor/LEI-derived DNase II	-	Sus scrofa

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Release 2023.1 (January 2023)