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Literature summary for 3.1.22.1 extracted from
- Purification and characterization of an acid deoxyribonuclease from the cultured mycelia of Cordyceps sinensis (2004), J. Biochem. Mol. Biol., 37, 466-473.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | above 200 mM | Ophiocordyceps sinensis |  |
| KCl | above 300 mM | Ophiocordyceps sinensis | |
| MgCl2 | above 150 mM | Ophiocordyceps sinensis | |
| MnCl2 | above 200 mM | Ophiocordyceps sinensis | |
| NaCl | above 300 mM | Ophiocordyceps sinensis | |
| ZnCl2 | above 150 mM | Ophiocordyceps sinensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Ophiocordyceps sinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ophiocordyceps sinensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ophiocordyceps sinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | cultured | Ophiocordyceps sinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ophiocordyceps sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | the enzyme acts on both dsDNA and ssDNA, dsDNA is preferred, the enzyme causes double-strand breaks in DNA substrates and generates 3-phosphate and 5-OH termini | Ophiocordyceps sinensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Ophiocordyceps sinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid deoxyribonuclease | - |
Ophiocordyceps sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Ophiocordyceps sinensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 70% of maximal activity, 70°C: about 50% of maximal activity | Ophiocordyceps sinensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Ophiocordyceps sinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | pH 4.0: about 30% of maximal activity, pH 7.5: about 45% of maximal activity | Ophiocordyceps sinensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ophiocordyceps sinensis | isoelectric focusing | - |
7.5 |
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