Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | protects the enzyme against traces of heavy metals | Sus scrofa | |
EDTA | activator | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinomycin D | strong inhibition, coupling to the substrate not to the enzyme | Sus scrofa | |
DNA | inhibitory above 0.4 mg/ml | Sus scrofa | |
HPO42- | slightly inhibitory above pH 5.0 | Sus scrofa | |
iodoacetamide | slight inhibitory | Sus scrofa | |
iodoacetate | strongly inhibitory | Sus scrofa | |
Mg2+ | slightly inhibitory at 10 mM and pH 4.5, inhibitory effects depending on enzyme purity | Sus scrofa | |
N-bromosuccinimide | strong inhibitory | Sus scrofa | |
Protein inhibitor | inhibition below pH 5.6, forming a DNase-inhibitor complex | Sus scrofa | |
rRNA | specific competitive inhibition | Sus scrofa | |
SO42- | very strong inhibition, particulary above pH 4.5 | Sus scrofa | |
succinate buffer | activity less than 3% of that in acetate buffer at pH 5.0 | Sus scrofa | |
tRNA | specific competitive inhibition | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Sus scrofa | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
- |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Sus scrofa | involvement in recombination, replication and degradation of DNA | oligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glucosamine and neutral sugar residues | Sus scrofa |
Purification (Comment) | Organism |
---|---|
near homogeneity by chromatography steps | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | multiple isoforms | Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis(p-nitrophenyl) phosphate + H2O | slow hydrolysis at optimal pH from 5.6 to 5.9 | Sus scrofa | p-nitrophenol + p-nitrophenyl phosphate: | - |
? | |
deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O | - |
Sus scrofa | p-nitrophenol + deoxyribonucleoside 5'-phosphates | - |
? | |
DNA + H2O | double-strand and single-strand scission | Sus scrofa | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | formation of oligonucleotides of an average chain length from 14 to 100 in the middle activity phase, purine nucleotides form about 75% of the 3'-terminals, formation of oligonucleotides of an average chain length from 6 to 14 in the terminal activity phase | ? | |
DNA + H2O | involvement in recombination, replication and degradation of DNA | Sus scrofa | oligonucleotides + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
at low ionic strength DNase is active at neutral pH | Sus scrofa |