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Literature summary for 3.1.22.1 extracted from

  • Bernarde, G.
    Spleen acid deoxyribonuclease (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 271-287.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
cysteine protects the enzyme against traces of heavy metals Sus scrofa
EDTA activator Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
actinomycin D strong inhibition, coupling to the substrate not to the enzyme Sus scrofa
DNA inhibitory above 0.4 mg/ml Sus scrofa
HPO42- slightly inhibitory above pH 5.0 Sus scrofa
iodoacetamide slight inhibitory Sus scrofa
iodoacetate strongly inhibitory Sus scrofa
Mg2+ slightly inhibitory at 10 mM and pH 4.5, inhibitory effects depending on enzyme purity Sus scrofa
N-bromosuccinimide strong inhibitory Sus scrofa
Protein inhibitor inhibition below pH 5.6, forming a DNase-inhibitor complex Sus scrofa
rRNA specific competitive inhibition Sus scrofa
SO42- very strong inhibition, particulary above pH 4.5 Sus scrofa
succinate buffer activity less than 3% of that in acetate buffer at pH 5.0 Sus scrofa
tRNA specific competitive inhibition Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Sus scrofa 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
-
Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Sus scrofa involvement in recombination, replication and degradation of DNA oligonucleotides + ?
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glucosamine and neutral sugar residues Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
near homogeneity by chromatography steps Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
spleen multiple isoforms Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(p-nitrophenyl) phosphate + H2O slow hydrolysis at optimal pH from 5.6 to 5.9 Sus scrofa p-nitrophenol + p-nitrophenyl phosphate:
-
?
deoxyribonucleoside 5'-phosphates p-nitrophenyl esters + H2O
-
Sus scrofa p-nitrophenol + deoxyribonucleoside 5'-phosphates
-
?
DNA + H2O double-strand and single-strand scission Sus scrofa 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides formation of oligonucleotides of an average chain length from 14 to 100 in the middle activity phase, purine nucleotides form about 75% of the 3'-terminals, formation of oligonucleotides of an average chain length from 6 to 14 in the terminal activity phase ?
DNA + H2O involvement in recombination, replication and degradation of DNA Sus scrofa oligonucleotides + ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
at low ionic strength DNase is active at neutral pH Sus scrofa