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Literature summary for 3.1.22.1 extracted from

  • Dulaney, J.T.; Touster, O.
    Isolation of deoxyribonuclease II of rat liver lysosomes (1972), J. Biol. Chem., 247, 1424-1432.
    View publication on PubMed
Search for more literature for 3.1.22.1

General Stability

General Stability Organism
addition of bovine serum albumin stabilizes enzyme activity at 37°C Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate 85% inhibition at 5 mM Rattus sp.
Mn(CH3COO)2 32% inhibition at 20 mM Rattus sp.
SO42- 68% inhibition at 20 mM Rattus sp.
Zn(CH3COO)2 67% inbhibition at 20 mM Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus sp. 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 gel filtration Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by sucrose centrifugation steps and chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Storage Stability

Storage Stability Organism
8°C, 0.4 M NaCl, 0.05 M acetate, pH 5.5, one third of activity after 1 year Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O calf thymus DNA, micrococcus luteus DNA and poly[d(A-T)d(A-T)] Rattus sp. 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides formation of dinucleotides to 8 nucleotide chain length ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1
-
-
 Rattus sp.