Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | complete inhibition at 0.1 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
near homogeneity by 5step chromatography techniques | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphoblast | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 7.4, 5 mM 2-mercaptoethanol, 1 mM EDTA, 1 mM PMSF, 50% glycerol v/v, several months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | preference for double-stranded DNA, terminal four 3'-nucleotides resistant to cleavage | Homo sapiens | 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides | prominent fragments longer than eight nucleotides | ? | |
supercoiled plasmid DNA + H2O | introduces a strong excess of single-strand nicks relative to double-strand breaks, more than 50% converted to nicked circular DNA | Homo sapiens | relaxed plasmid DNA + ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
irreversible loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Homo sapiens |