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Literature summary for 3.1.21.9 extracted from
- T4 endonuclease IV. Improved purification procedure and resolution from T4 endonuclease 3 (1972), J. Biol. Chem., 247, 405-412.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tequatrovirus T4 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 840000 | - |
pH 7.5, 37°C | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme displays about 200fold greater activity toward single stranded DNA than toward duplex DNA. The limit product obtained by exhaustive digestion of single stranded DNA with endonuclease IV is 25% acid-soluble and consists of oligonucleotides with an average chain length of about 50 nucleotides. Enzyme does not display exonuclease activity. No substrate: denatured bacteriophage T4 DNA | Tequatrovirus T4 | ? | - |
? |  |
| phage fd DNA + H2O | - |
Tequatrovirus T4 | ? | - |
? | |
| polydeoxycytydilate + H2O | - |
Tequatrovirus T4 | ? | product is acid-soluble material ending excluively in 5'-dCMP | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
- |
Tequatrovirus T4 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
62% of maximum activity | Tequatrovirus T4 |
| 8.5 | - |
62% of maximum activity | Tequatrovirus T4 |
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Release 2023.1 (January 2023)
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