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Literature summary for 3.1.21.9 extracted from

  • Sadowski, P.D.; Hurwitz, J.
    Enzymatic breakage of deoxyribonucleic acid. II. Purification and properties of endonuclease IV from T4 phage-infected Escherichia coli (1969), J. Biol. Chem., 244, 6192-6198.
    View publication on PubMed
Search for more literature for 3.1.21.9

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 0.05 M, 70% inhibition Tequatrovirus T4
K3PO4 0.05 M, 80% inhibition Tequatrovirus T4
KCl 0.05 M, 80% inhibition Tequatrovirus T4
NaCl 0.05 M, 85% inhibition Tequatrovirus T4
NH4Cl 0.05 M, 47% inhibition Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ CoCl2 can substitute for MgCl2. The activity at the optimal Co2+ concentration (0.001 M) is 27% higher than that found at the optimal Mg2+ concentration Tequatrovirus T4
Mg2+ optimal concentration is 0.01 M. The activity at 0.001 M and 0.05 M MgCl2 is 50% of that at 0.01 M Tequatrovirus T4
Mn2+ Mn2+ (0.001 M) quantitatively can replace Mg2+ in the reaction Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4 the phage T4 enzyme is involved in degradation of host DNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified about 150fold from T4 phage-infected Escherichia coli B Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4 ?
-
 ?
DNA + H2O the phage T4 enzyme is involved in degradation of host DNA. It introduces breaks preferentially into single stranded DNA. It produces oligonucleotides as small as 150 nucleotides long from fd DNA. These oligonucleotides contain 3'-hydroxyl and 5'-phosphate termini. T4 endonuclease IV produces breaks exclusively adjacent to cytosine residues in both fd and denatured lambda DNA.The enzyme does not cleave double-stranded DNA or native T4 DNA, which contains 5-hydroxymethylcytosine instead of cytosine Tequatrovirus T4 ?
-
 ?

Synonyms

Synonyms Comment Organism
denB
-
 Tequatrovirus T4
EndoIV ambiguous Tequatrovirus T4
T4 endonuclease IV
-
 Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Tequatrovirus T4
8.4 9.2 measured in 0.05 M Tris or glycine buffer Tequatrovirus T4

General Information

General Information Comment Organism
physiological function the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4