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Literature summary for 3.1.21.8 extracted from

  • Carlson, K.; Kosturko, L.D.; Nystrom, A.C.
    Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro (1999), Mol. Microbiol., 31, 1395-1405.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ above 10 mM Tequatrovirus T4
NaCl above 50 mM Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Tequatrovirus T4 the phage T4 enzyme is involved in degradation of host DNA ?
-
?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P07059
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4 ?
-
?
DNA + H2O the phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5fold to 10fold less frequently. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine Tequatrovirus T4 ?
-
?

Synonyms

Synonyms Comment Organism
denA
-
Tequatrovirus T4
EndoII ambiguous Tequatrovirus T4
T4 endonuclease II
-
Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37 activity at 30°C is somewhat lower than at 37°C Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
enzyme remains active for at least 120 min Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
Tequatrovirus T4

General Information

General Information Comment Organism
physiological function the phage T4 enzyme is involved in degradation of host DNA Tequatrovirus T4