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Literature summary for 3.1.21.7 extracted from

  • Garcia-Caballero, D.; Perez-Moreno, G.; Estevez, A.M.; Ruiz-Perez, L.M.; Vidal, A.E.; Gonzalez-Pacanowska, D.
    Insights into the role of endonuclease V in RNA metabolism in Trypanosoma brucei (2017), Sci. Rep., 7, 8505 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.7

Cloned(Commentary)

Cloned (Comment) Organism
the MBP-TbEndoV protein is overexpressed in the Escherichia coli TB1 strain Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
KCl above 100 mM, strong inhibition Trypanosoma brucei brucei
NaCl above 100 mM, strong inhibition Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol the enzyme is mainly cytosolic yet upon nutritional stress it redistributes and accumulates in stress granules colocalizing with the DEAD-box helicase TbDhh1 Trypanosoma brucei 5829
-
cytosol the enzyme is mainly cytosolic yet upon nutritional stress it redistributes and accumulates in stress granules colocalizing with the DEAD-box helicase TbDhh1 Trypanosoma brucei brucei 5829
-
stress granule the enzyme is mainly cytosolic yet upon nutritional stress it redistributes and accumulates in stress granules colocalizing with the DEAD-box helicase TbDhh1 Trypanosoma brucei
-
-
stress granule the enzyme is mainly cytosolic yet upon nutritional stress it redistributes and accumulates in stress granules colocalizing with the DEAD-box helicase TbDhh1 Trypanosoma brucei brucei
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ high activity at pH 7.5 when using 1 mM MnCl2 Trypanosoma brucei brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34922
-
 calculated from sequence Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
single-stranded RNA + H2O Trypanosoma brucei in prokaryotes, endonuclease V (EndoV) can recognize and cleave inosine-containing DNA. In contrast, mammalian EndoVs preferentially cleave inosine-containing RNA, suggesting a role in RNA metabolism for the eukaryotic members of this protein family ?
-
 ?
single-stranded RNA + H2O Trypanosoma brucei 927 in prokaryotes, endonuclease V (EndoV) can recognize and cleave inosine-containing DNA. In contrast, mammalian EndoVs preferentially cleave inosine-containing RNA, suggesting a role in RNA metabolism for the eukaryotic members of this protein family ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei Q38AS1
-
-
Trypanosoma brucei 927 Q38AS1
-
-
Trypanosoma brucei brucei Q38AS1
-
-
Trypanosoma brucei brucei 927/4 GUTat10.1 Q38AS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma brucei
-
 Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA-like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei ?
-
 ?
DNA + H2O the enzyme efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA-like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei 927 ?
-
 ?
single-stranded RNA + H2O in prokaryotes, endonuclease V (EndoV) can recognize and cleave inosine-containing DNA. In contrast, mammalian EndoVs preferentially cleave inosine-containing RNA, suggesting a role in RNA metabolism for the eukaryotic members of this protein family Trypanosoma brucei ?
-
 ?
single-stranded RNA + H2O the enzyme efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA-like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei ?
-
 ?
single-stranded RNA + H2O in prokaryotes, endonuclease V (EndoV) can recognize and cleave inosine-containing DNA. In contrast, mammalian EndoVs preferentially cleave inosine-containing RNA, suggesting a role in RNA metabolism for the eukaryotic members of this protein family Trypanosoma brucei 927 ?
-
 ?
single-stranded RNA + H2O the enzyme efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA-like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei 927 ?
-
 ?
ssRNA rI + H2O efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei brucei ?
-
 ?
ssRNA rI + H2O efficiently processes single-stranded RNA oligonucleotides with inosine, including A to I-edited tRNA like substrates but exhibits weak activity over DNA, except when a ribonucleotide is placed 3' to the inosine Trypanosoma brucei brucei 927/4 GUTat10.1 ?
-
 ?

Synonyms

Synonyms Comment Organism
EndoV
-
 Trypanosoma brucei brucei
Tb10.6k15.3890
-
 Trypanosoma brucei

General Information

General Information Comment Organism
malfunction RNAi-mediated depletion of TbEndoV results in moderate growth defects in procyclic cells while the two EndoV alleles can be readily knocked out in bloodstream forms Trypanosoma brucei
malfunction RNAi-mediated depletion of TbEndoV results in moderate growth defects in procyclic cells while the two EndoV alleles can be readily knocked out in bloodstream forms. The enzyme has an important role in RNA metabolism in procyclic forms of the parasite Trypanosoma brucei brucei
physiological function endonuclease V has an important role in RNA metabolism in procyclic forms of the parasite Trypanosoma brucei