Literature summary for 3.1.21.7 extracted from

Nawaz, M.S.; Vik, E.S.; Ronander, M.E.; Solvoll, A.M.; Blicher, P.; Bjoras, M.; Alseth, I.; Dalhus, B.
Crystal structure and MD simulation of mouse EndoV reveal wedge motif plasticity in this inosine-specific endonuclease (2016), Sci. Rep., 6, 24979 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in a modified version of Escherichia coli ER 2566 (ER2566DELTAnfiDELTArnhB) where the endogenous nfi and rnhB genes have been inactivated by transformation with the kanamycin-deletion cassettes derived from the JW5547-1 (nfi::kan) and JW0178-1 (rnhB::kan) strains. For crystallisation, a site specific (D52A) truncated (residues 1-250) mutant of mEndoV is fused to His/MBP in pETM41 is expressed in BL21 CodonPlus(DE3)-RIPL Escherichia coli cells	Mus musculus

Cloned (Comment)

Organism

expressed in a modified version of Escherichia coli ER 2566 (ER2566DELTAnfiDELTArnhB) where the endogenous nfi and rnhB genes have been inactivated by transformation with the kanamycin-deletion cassettes derived from the JW5547-1 (nfi::kan) and JW0178-1 (rnhB::kan) strains. For crystallisation, a site specific (D52A) truncated (residues 1-250) mutant of mEndoV is fused to His/MBP in pETM41 is expressed in BL21 CodonPlus(DE3)-RIPL Escherichia coli cells

Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
D52A	mutant enzyme totally loses the inosine-RNA incision activity	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
Mn2+	at pH 8.5, a Mn2+ concentration of 2.5 mM and higher inhibits activity	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	best activity at pH 8.5 and 2.5 mM Mg2+	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8C9A2	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ACUGGACA[rI][rI]U[rI]CUCCGAGG + H2O	-	Mus musculus	?	-	?
RNA + H2O	the enzyme is active on RNA substrates containing one or several inosines. It does not cleave at inosines in DNA	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
EndoV	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0026	-	ACUGGACA[rI][rI]U[rI]CUCCGAGG	pH 7.5, 37°C	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Mus musculus