Literature summary for 3.1.21.7 extracted from

Nawaz, M.S.; Vik, E.S.; Berges, N.; Fladeby, C.; Bjoras, M.; Dalhus, B.; Alseth, I.
Regulation of human endonuclease V activity and relocalization to cytoplasmic stress granules (2016), J. Biol. Chem., 291, 21786-21801 .

Search for more literature for 3.1.21.7

Activating Compound

Activating Compound	Comment	Organism	Structure
polyadenylate-binding protein C1	the association between polyadenylate-binding protein C1 and hEndoV is RNA dependent. Polyadenylate-binding protein stimulates hEndoV activity and affinity for inosine-containing RNA. Upon cellular stress, polyadenylate-binding protein C1 relocates to cytoplasmic stress granules	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
HEK 293T cells are transiently transfected with a vector expressing GFP-hEndoVwt	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
ATP	1 mM ATP almost completely inhibits hEndoV activity. hEndoV cleavage is inhibited by normal intracellular ATP concentrations. The ATP stores inside a cell do not overlay stress granules and it is suggest that hEndoV is redistributed to stress granules as a strategy to create a local environment low in ATP to permit hEndoV activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	hEndoV both have a cytoplasmic localization. It relocates to cytoplasmic stress granules in cultured human cells upon exposure to various toxic agents. This is a mechanism for the cell to regulate hEndoV activity	Homo sapiens	5737	-
stress granule	the enzyme relocates to cytoplasmic stress granules in cultured human cells upon exposure to various toxic agents. This is a mechanism for the cell to regulate hEndoV activity	Homo sapiens	-	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8N8Q3	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HeLa cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RNA + H2O	the enzyme cleaves RNA with inosine but not DNA	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
EndoV	-	Homo sapiens

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Release 2023.1 (January 2023)