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Literature summary for 3.1.21.7 extracted from

  • Zhang, Z.; Hao, Z.; Wang, Z.; Li, Q.; Xie, W.
    Structure of human endonuclease V as an inosine-specific ribonuclease (2014), Acta Crystallogr. Sect. D, 70, 2286-2294.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 19% (w/v) PEG 3350, 0.2 M sodium formate, 0.1 M Tris-HCl pH 7.5 or 1.4 M ammonium sulfate, 0.2 M sodium acetate, 0.1 M Tris-HCl pH 7.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27800
-
1 * 27800, calculated from amino acid sequence Homo sapiens
28800
-
1 * 28800, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Homo sapiens 5'-GCTCGGCTICGGACCGAG-3'. Endonuclease V is an inosine-specific nucleases that cleave at the second phosphodiester bond 3' to inosine ?
-
?
single-stranded RNA + H2O Homo sapiens 5'-CUGACUICGGAUCAGGGCC-3'. The enzyme is most active towards single-stranded RNA but is much less active towards other substrates ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N8Q3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O 5'-GCTCGGCTICGGACCGAG-3'. Endonuclease V is an inosine-specific nucleases that cleave at the second phosphodiester bond 3' to inosine Homo sapiens ?
-
?
single-stranded RNA + H2O 5'-CUGACUICGGAUCAGGGCC-3'. The enzyme is most active towards single-stranded RNA but is much less active towards other substrates Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27800, calculated from amino acid sequence Homo sapiens
monomer 1 * 28800, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
endonuclease V
-
Homo sapiens
EndoV
-
Homo sapiens