Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 CodonPlus (DE3) RIL | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
EndoV in complex with a hypoxanthine lesion substrate and with product DNA, hanging drop vapour diffusion method, in 6-12% (w/v) MPEG 2k in 200 mM imidazole-matate buffer, pH 5.8-7.4 | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D43A | the mutant is unable to bind the Mg2+ cofactor | Thermotoga maritima |
Y80A | the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product | Thermotoga maritima |
Y80F | the mutant is similar to the wild type EndoV in its ability to bind to DNA base lesions and the corresponding nicked product | Thermotoga maritima |
Y80H | the mutant is severely compromised in its ability to bind to DNA base lesions and the corresponding nicked product | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | contains one Mg2+ ion in the active site, which is required for activity | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X2H9 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap SP XL column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | EndoV hydrolyzes the second phosphodiester bond 3' of a deaminated base using Mg2+ as a cofactor | Thermotoga maritima | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
additional information | EndoV cleaves substrates with base mismatches and helical distortions, such as mismatch loops, hairpins and flap structures, EndoV cleaves mismatched base pairs preferentially at adenine and guanine purines, EndoV binds to cleaved mismatch base pair products with much lower affinity as compared to cleaved deaminated bases | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease V | - |
Thermotoga maritima |
EndoV | - |
Thermotoga maritima |