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Literature summary for 3.1.21.7 extracted from

  • Miyazaki, K.
    Random DNA fragmentation with enonuclease V: application to DNA shuffling (2002), Nucleic Acids Res., 30, e139.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
molecular biology application to DNA shuffling. Random DNA fragmentation by endonuclease V is a handy and reproducible method and can be used instead of fragmentation by DNase I, which is technically problematic due to decreasing shuffling efficiency Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme nicks uracil-containing DNA at the seond or third phosphodiester bond 3' to uracil sites Escherichia coli ?
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