Literature summary for 3.1.21.7 extracted from

Latham, K.A.; Lloyd, R.S.
delta-Eliminiation by T4 endonuclease V at a thymine dimer site requires a secondary binding event and amino acid Glu-23 (1995), Biochemistry, 34, 8796-8803.

Search for more literature for 3.1.21.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Tequatrovirus T4

Protein Variants

Protein Variants	Comment	Organism
E23Q	required for abasic lyase activity	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, chromatography steps, recombinant enzymes	Tequatrovirus T4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
49-mer oligonucleotide + H2O	also possesses abasic lyase activity	Tequatrovirus T4	hydrolysed DNA	-	?
49-mer oligonucleotide + H2O	double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer, enzyme requires a secondary binding event after beta-elimination at a pyrimidine dimer or abasic site	Tequatrovirus T4	3-alpha,beta-unsaturated aldehyde + 3'-phosphate DNA-termini	delta-elimination reaction	?

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Release 2023.1 (January 2023)