Literature summary for 3.1.21.5 extracted from

Ahmad, I.; Kulkarni, M.; Gopinath, A.; Saikrishnan, K.
Single-site DNA cleavage by Type III restriction endonuclease requires a site-bound enzyme and a trans-acting enzyme that are ATPase-activated (2018), Nucleic Acids Res., 46, 6229-6237 .

Search for more literature for 3.1.21.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3) cells	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q5ZND2	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	single-site cleavage is solely a result of the interaction between two independent enzymes: a cis-acting enzyme which is bound to its recognition site in the DNA and a trans-acting enzyme either bound to or activated by its substrate in ATP-dependent manner. The two cooperating enzymes in solution can come together via diffusion. For cleavage to happen, the interaction between the two nucleases should occur within a time period shorter than the time required by the enzyme in cis to leave its recognition site. In case of EcoP15I, this time is measured to be about 6-17 seconds. According to a model of DNA cleavage a recognition site bound Type III RM enzyme would undergo a conformational change induced by the hydrolysis of ATP making it both diffusion-competent and nucleolytically active. The activated nuclease can catalyze single-strand scission only in cooperation with another ATP-activated nuclease. It is proposed that this model is valid for both single-site and two-site cleavage, except that in single-site cleavage the cooperating nucleases come together by 3D diffusion, and in two-site cleavage they converge by 1D diffusion and/or looping	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
EcoP15I	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	Type III RM enzymes are bacterial defense systems that protect the host from invading foreign DNA by nucleolytically cleaving them at specific recognition sites	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)