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Literature summary for 3.1.21.5 extracted from

  • Corvaglia, A.R.; Francois, P.; Hernandez, D.; Perron, K.; Linder, P.; Schrenzel, J.
    A type III-like restriction endonuclease functions as a major barrier to horizontal gene transfer in clinical Staphylococcus aureus strains (2010), Proc. Natl. Acad. Sci. USA, 107, 11954-11958.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information inactivation of this restriction system dramatically increases the transformation efficiency of clinical Staphylococcus aureus methicillin-resistant strains, opening the field of molecular genetic manipulation of these strains using DNA of exogenous origin Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Staphylococcus aureus
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different strains
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information EcoP15I recognizes 5'-CAGCAG-3' as target site. Cleavage occurs at a defined location next to only one of the sites, and one of the two sites is chosen for cleavage randomly, although this is influenced by the base composition of the DNA. The EcoP15I R/M enzyme cuts the sequence CAGCAG(N)25/27 as long as the underlined adenine is unmethylated. Preference for a two-site substrate. ATP hydrolysis is essential for the overall restriction process Escherichia coli ?
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?

Subunits

Subunits Comment Organism
More the type III R/M enzymes comprise two modification subunits, each containing a target recognition domain to bind to the target sequence and a methyltransferase catalytic domain to monitor the methylation status of an adenine in the target, and two restriction subunits each containing a DNA helicase and ATP-hydrolysing domain and an endonuclease DNA cleavage domain Escherichia coli

Synonyms

Synonyms Comment Organism
ATP-dependent type III restriction endonuclease
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Escherichia coli
EcoP15I
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Escherichia coli
type III DNA restriction/modification enzyme
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Escherichia coli
type III R/M enzyme
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Escherichia coli
type III restriction enzyme
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Escherichia coli
type III-like restriction endonuclease
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Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP ATP-dependent type III restriction endonuclease Escherichia coli

General Information

General Information Comment Organism
additional information several models for the mode of action of type III R/M enzymes, detailed overview. 1. Translocation, loop extrusion and collision model. 2. The end reversal model. 3. Transient looping and translocation model from atomic force spectroscopy Escherichia coli
physiological function characterization of a type III-like restriction system present in clinical Staphylococcus aureus strains that prevents transformation with DNA from other bacterial species. Some methicillin-resistant strains are deficient in this restriction system, and thus are hypersusceptible to the horizontal transfer of DNA from other species, such as Escherichia coli, and could easily acquire a vancomycin-resistance gene from Enterococci, overview Staphylococcus aureus