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Literature summary for 3.1.21.5 extracted from
- Unidirectional translocation from recognition site and a necessary interaction with DNA end for cleavage by Type III restriction enzyme (2004), Nucleic Acids Res., 32, 5703-5711.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HU protein | binding of HU protein interfers with R.EcoP15I cleavage activity | Escherichia coli | |
| Lac repressor | cleavage can be abolished by the binding of Lac repressor downstream (3' side) but not upstream (5' side) of the recognition site | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | cleavage site does not depend on the sequence context of the recognition sie. The enzyme can cleave linear DNA having either recognition sites in the same orientation or a single recognition site. Cleavage occurs predominantly at a site proximal to the DNA end in the case of multiple site substrates. The mechanism requires two enzyme molecules cooperating to elicit double-stranded break on DNA. The enzyme translocates on DNA in a 5' to 3' direction from its recognition site, switch in the direction of enzyme motion at the FNA ends | Escherichia coli | specific double-stranded DNA fragments with terminal 5'-phosphate | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| R.EcoP15I | - |
Escherichia coli |
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