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Literature summary for 3.1.21.5 extracted from

  • Peakman, L.J.; Antognozzi, M.; Bickle, T.A.; Janscak, P.; Szczelkun, M.D.
    S-adenosyl methionine prevents promiscuous DNA cleavage by the EcoP1I type III restriction enzyme (2003), J. Mol. Biol., 333, 321-335.
    View publication on PubMed
Search for more literature for 3.1.21.5

Organism

Organism UniProt Comment Textmining
prophage P1
-
 enzyme EcoP1I
-

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates mechanism prophage P1
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cleavage generally depends on ratio of enzyme to cleavage sites, in presence of S-adenosyl-L-methionine, substrates with two cleavage sites in inverted repeats are susceptible, in absence of S-adenosyl-L-methionine, presence of Na+ leads to cleavage only in substrates with two sites in inverted repeats, presence of K+ leads to cleavage of all substrates prophage P1 ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine allosteric activator and specificity factor that ensures that cleavage only occurs when two endonucleases bind two recognition sites in a designated orientation prophage P1