Literature summary for 3.1.21.4 extracted from

Callahan, S.J.; Luyten, Y.A.; Gupta, Y.K.; Wilson, G.G.; Roberts, R.J.; Morgan, R.D.; Aggarwal, A.K.
Structure of type IIL restriction-modification enzyme MmeI in complex with DNA has implications for engineering new specificities (2016), PLoS Biol., 14, e1002471 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of MmeI in complex with its DNA substrate (a 29-mer DNA duplex containing a single MmeI recognition site (TCCGAC)) and an S-adenosylmethionine analog sinefungin. The co-crystals are obtained in the presence of sinefungin and diffracted to 2.6 A resolution with synchrotron radiation. They belong to space group P1 with unit cell dimensions of a = 61.87 A, b = 95.29 A, c = 161.96 A, alpha = 72.84°, beta = 89.15°, and gamma = 71.61°, and contain two MmeI/DNA/sinefungin complexes in the crystallographic asymmetric unit	Methylophilus methylotrophus

Crystallization (Comment)

Organism

crystal structure of MmeI in complex with its DNA substrate (a 29-mer DNA duplex containing a single MmeI recognition site (TCCGAC)) and an S-adenosylmethionine analog sinefungin. The co-crystals are obtained in the presence of sinefungin and diffracted to 2.6 A resolution with synchrotron radiation. They belong to space group P1 with unit cell dimensions of a = 61.87 A, b = 95.29 A, c = 161.96 A, alpha = 72.84°, beta = 89.15°, and gamma = 71.61°, and contain two MmeI/DNA/sinefungin complexes in the crystallographic asymmetric unit

Methylophilus methylotrophus

Inhibitors

Inhibitors	Comment	Organism	Structure
sinefungin	-	Methylophilus methylotrophus

Organism

Organism	UniProt	Comment	Textmining
Methylophilus methylotrophus	B2MU09	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methylophilus methylotrophus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	the enzyme is resilient to specificity changes at the first position of the recognition sequence (5'-TCCRAC-3')	Methylophilus methylotrophus	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Methylophilus methylotrophus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	assay at	Methylophilus methylotrophus

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Release 2023.1 (January 2023)