Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method at 19°C. 1.6 A co-crystal structure of UbaLAI N-terminal domain | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | A0A291ATB4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | the enzyme is specific for the pseudosymmetric DNA sequence 5'-CC/WGG-3' (where W = A/T, and / marks the cleavage position). UbaLAI requires two recognition sites for optimal activity, and, like other type IIE enzymes, uses one copy of a recognition site to stimulate cleavage of a second copy. It is proposed that during the reaction the UbaLAI N-terminal domain acts as a handle that tethers the monomeric UbaLAI C-terminal domain to the DNA, thereby helping UbaLAI-C to perform two sequential DNA nicking reactions on the second recognition site during a single DNA-binding event | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
UbaLAI | - |
uncultured bacterium |