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Literature summary for 3.1.21.4 extracted from

  • Sasnauskas, G.; Tamulaitiene, G.; Tamulaitis, G.; Calyseva, J.; Laime, M.; Rimsellene, R.; Lubys, A.; Siksnys, V.
    UbaLAI is a monomeric Type IIE restriction enzyme (2017), Nucleic Acids Res., 45, 9583-9594 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 19°C. 1.6 A co-crystal structure of UbaLAI N-terminal domain uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium A0A291ATB4
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme is specific for the pseudosymmetric DNA sequence 5'-CC/WGG-3' (where W = A/T, and / marks the cleavage position). UbaLAI requires two recognition sites for optimal activity, and, like other type IIE enzymes, uses one copy of a recognition site to stimulate cleavage of a second copy. It is proposed that during the reaction the UbaLAI N-terminal domain acts as a handle that tethers the monomeric UbaLAI C-terminal domain to the DNA, thereby helping UbaLAI-C to perform two sequential DNA nicking reactions on the second recognition site during a single DNA-binding event uncultured bacterium ?
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Subunits

Subunits Comment Organism
monomer
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uncultured bacterium

Synonyms

Synonyms Comment Organism
UbaLAI
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uncultured bacterium