Literature summary for 3.1.21.4 extracted from

Tamulaitis, G.; Rutkauskas, M.; Zaremba, M.; Grazulis, S.; Tamulaitiene, G.; Siksnys, V.
Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI (2015), Nucleic Acids Res., 43, 8100-8110 .

Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method at 19°C	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q6UQ65	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	the enzyme recognizes a degenerated sequence 5'-W/CCGGW-3' (W stands for A or T and / denotes the cleavage site). It belongs to a large family of restriction enzymes that contain a conserved CCGG tetranucleotide in their target sites. It requires binding of two target sites for the optimal catalytic activity	Geobacillus stearothermophilus	?	-	?

Subunits

Subunits	Comment	Organism
dimer	in solution the BsaWI protein exists in a dimer-tetramer-oligomer equilibrium, but in the presence of specific DNA forms a tetramer bound to two target sites. Site-directed mutagenesis and kinetic experiments show that BsaWI is active as a tetramer and requires two target sites for optimal activity	Geobacillus stearothermophilus
tetramer	in solution the BsaWI protein exists in a dimer-tetramer-oligomer equilibrium, but in the presence of specific DNA forms a tetramer bound to two target sites. Site-directed mutagenesis and kinetic experiments show that BsaWI is active as a tetramer and requires two target sites for optimal activity	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
BsaWI	-	Geobacillus stearothermophilus
type IIF restriction endonuclease	-	Geobacillus stearothermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)