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Literature summary for 3.1.21.4 extracted from

  • Marshall, J.J.; Halford, S.E.
    The type IIB restriction endonucleases (2010), Biochem. Soc. Trans., 38, 410-416.
    View publication on PubMed
Search for more literature for 3.1.21.4

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine required Geobacillus stearothermophilus
S-adenosyl-L-methionine required Lysinibacillus sphaericus
S-adenosyl-L-methionine required Weizmannia coagulans
S-adenosyl-L-methionine required Bacillus pumilus
S-adenosyl-L-methionine required Citrobacter sp.
S-adenosyl-L-methionine required Acinetobacter lwoffii
S-adenosyl-L-methionine required Campylobacter jejuni

Application

Application Comment Organism
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Geobacillus stearothermophilus
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Lysinibacillus sphaericus
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Pseudomonas putida
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Neisseria meningitidis
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Weizmannia coagulans
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Bacillus pumilus
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Citrobacter sp.
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Haemophilus aegyptius
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Acinetobacter lwoffii
analysis type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Campylobacter jejuni
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Geobacillus stearothermophilus
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Lysinibacillus sphaericus
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Pseudomonas putida
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Neisseria meningitidis
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Weizmannia coagulans
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Bacillus pumilus
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Citrobacter sp.
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Haemophilus aegyptius
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Acinetobacter lwoffii
molecular biology type II REases are widely used as tools for the dissection, analysis and reconstruction of DNA Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Weizmannia coagulans the enzyme has the recognition sequence (10/12) CGAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Acinetobacter lwoffii the enzyme has the recognition sequence (10/12) GCAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Lysinibacillus sphaericus the enzyme has the recognition sequence (10/15) ACN4GTAYC (12/7), of which it needs 2 on the substrate to be active. It excises 28 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Citrobacter sp. the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Neisseria meningitidis the enzyme has the recognition sequence (12/7) RCCGGY (7/12), of which it needs 2 on the substrate to be active. It excises 20 bp, and does not require S-adenosyl-L-methionine ?
-
 ?
additional information Pseudomonas putida the enzyme has the recognition sequence (7/12) GAACN6CTC (13/8), of which it needs 2 on the substrate to be active. It excises 28 bp, and does not require S-adenosyl-L-methionine ?
-
 ?
additional information Acinetobacter lwoffii the enzyme has the recognition sequence (7/12) GAACN6TCC (12/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and does not require S-adenosyl-L-methionine ?
-
 ?
additional information Haemophilus aegyptius the enzyme has the recognition sequence (7/13) GAYN5RTC (14/9). It excises 27 bp, and does not require S-adenosyl-L-methionine ?
-
 ?
additional information Bacillus pumilus the enzyme has the recognition sequence (8/13) GAGN5CTC (13/8), of which it needs 1 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Campylobacter jejuni the enzyme has the recognition sequence (8/14) CCAN6GT (15/9): It excises 28 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Geobacillus stearothermophilus the enzyme has the recognition sequence (9/12) ACN5CTCC (10/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine ?
-
 ?
additional information Citrobacter sp. 2144 the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter lwoffii
-
-
-
Bacillus pumilus
-
-
-
Campylobacter jejuni
-
-
-
Citrobacter sp.
-
-
-
Citrobacter sp. 2144
-
-
-
Geobacillus stearothermophilus
-
-
-
Haemophilus aegyptius
-
-
-
Lysinibacillus sphaericus
-
-
-
Neisseria meningitidis
-
-
-
Pseudomonas putida
-
-
-
Weizmannia coagulans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has the recognition sequence (10/12) CGAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine Weizmannia coagulans ?
-
 ?
additional information the enzyme has the recognition sequence (10/12) GCAN6TGC (12/10), of which it needs 2 on the substrate to be active. It excises 32 bp, and requires S-adenosyl-L-methionine Acinetobacter lwoffii ?
-
 ?
additional information the enzyme has the recognition sequence (10/15) ACN4GTAYC (12/7), of which it needs 2 on the substrate to be active. It excises 28 bp, and requires S-adenosyl-L-methionine Lysinibacillus sphaericus ?
-
 ?
additional information the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine Citrobacter sp. ?
-
 ?
additional information the enzyme has the recognition sequence (12/7) RCCGGY (7/12), of which it needs 2 on the substrate to be active. It excises 20 bp, and does not require S-adenosyl-L-methionine Neisseria meningitidis ?
-
 ?
additional information the enzyme has the recognition sequence (7/12) GAACN6CTC (13/8), of which it needs 2 on the substrate to be active. It excises 28 bp, and does not require S-adenosyl-L-methionine Pseudomonas putida ?
-
 ?
additional information the enzyme has the recognition sequence (7/12) GAACN6TCC (12/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and does not require S-adenosyl-L-methionine Acinetobacter lwoffii ?
-
 ?
additional information the enzyme has the recognition sequence (7/13) GAYN5RTC (14/9). It excises 27 bp, and does not require S-adenosyl-L-methionine Haemophilus aegyptius ?
-
 ?
additional information the enzyme has the recognition sequence (8/13) GAGN5CTC (13/8), of which it needs 1 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine Bacillus pumilus ?
-
 ?
additional information the enzyme has the recognition sequence (8/14) CCAN6GT (15/9): It excises 28 bp, and requires S-adenosyl-L-methionine Campylobacter jejuni ?
-
 ?
additional information the enzyme has the recognition sequence (9/12) ACN5CTCC (10/7), of which it needs 2 on the substrate to be active. It excises 27 bp, and requires S-adenosyl-L-methionine Geobacillus stearothermophilus ?
-
 ?
additional information the enzyme has the recognition sequence (11/13) CAAN5GTGG (12/10), of which it needs 2 on the substrate to be active. It excises 33 bp, and requires S-adenosyl-L-methionine Citrobacter sp. 2144 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Lysinibacillus sphaericus
heterodimer
-
 Weizmannia coagulans
heterodimer
-
 Bacillus pumilus
monomer
-
 Pseudomonas putida
monomer
-
 Haemophilus aegyptius
monomer
-
 Acinetobacter lwoffii
monomer
-
 Campylobacter jejuni
More consists of two different polypeptide chains R and M Neisseria meningitidis

Synonyms

Synonyms Comment Organism
AlfI
-
 Acinetobacter lwoffii
AloI
-
 Acinetobacter lwoffii
BaeI
-
 Lysinibacillus sphaericus
BcgI
-
 Weizmannia coagulans
BplI
-
 Bacillus pumilus
BsaXI
-
 Geobacillus stearothermophilus
CjeI
-
 Campylobacter jejuni
CspCI
-
 Citrobacter sp.
HaeVI
-
 Haemophilus aegyptius
NmeDI
-
 Neisseria meningitidis
PPII
-
 Pseudomonas putida
type II REase
-
 Geobacillus stearothermophilus
type II REase
-
 Lysinibacillus sphaericus
type II REase
-
 Pseudomonas putida
type II REase
-
 Neisseria meningitidis
type II REase
-
 Weizmannia coagulans
type II REase
-
 Bacillus pumilus
type II REase
-
 Citrobacter sp.
type II REase
-
 Haemophilus aegyptius
type II REase
-
 Acinetobacter lwoffii
type II REase
-
 Campylobacter jejuni
type IIB restriction endonuclease
-
 Geobacillus stearothermophilus
type IIB restriction endonuclease
-
 Lysinibacillus sphaericus
type IIB restriction endonuclease
-
 Pseudomonas putida
type IIB restriction endonuclease
-
 Neisseria meningitidis
type IIB restriction endonuclease
-
 Weizmannia coagulans
type IIB restriction endonuclease
-
 Bacillus pumilus
type IIB restriction endonuclease
-
 Citrobacter sp.
type IIB restriction endonuclease
-
 Haemophilus aegyptius
type IIB restriction endonuclease
-
 Acinetobacter lwoffii
type IIB restriction endonuclease
-
 Campylobacter jejuni

General Information

General Information Comment Organism
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Geobacillus stearothermophilus
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Lysinibacillus sphaericus
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Pseudomonas putida
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Neisseria meningitidis
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Weizmannia coagulans
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Bacillus pumilus
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Citrobacter sp.
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Haemophilus aegyptius
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Acinetobacter lwoffii
evolution the fact that these enzymes cut DNA at specific locations mark them as type II systems, as opposed to the type I enzymes that cut DNA randomly, but in terms of gene organization and protein assembly, most type IIB restriction-modification systems have more in common with type I than with other type II systems Campylobacter jejuni
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Geobacillus stearothermophilus
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Lysinibacillus sphaericus
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Pseudomonas putida
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Neisseria meningitidis
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Weizmannia coagulans
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Bacillus pumilus
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Citrobacter sp.
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Haemophilus aegyptius
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Acinetobacter lwoffii
additional information reaction mode of type IIB enzyme in one or two polypeptide systems, overview Campylobacter jejuni
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Geobacillus stearothermophilus
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Lysinibacillus sphaericus
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Pseudomonas putida
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Neisseria meningitidis
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Weizmannia coagulans
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Bacillus pumilus
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Citrobacter sp.
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Haemophilus aegyptius
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Acinetobacter lwoffii
physiological function the endonucleases from the type IIB restriction-modification systems differ from all other restriction enzymes. The type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex Campylobacter jejuni