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Literature summary for 3.1.21.4 extracted from

  • Jakubauskas, A.; Giedriene, J.; Bujnicki, J.M.; Janulaitis, A.
    Identification of a single HNH active site in type IIS restriction endonuclease Eco31I (2007), J. Mol. Biol., 370, 157-169.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Escherichia coli
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isoform Eco31I
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Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates residues D311 and N334 coordinate the cofactor. H312 acts as a general base actiating a water molecule for the nucleophilic attack. K337 together with R340 and D345 are located close to the active center and are essential for correct folding of cataloytic motif, while D345, R264 and D273 may be directly involved in DNA binding Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O DNA recognition site is GTCTC Escherichia coli ?
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?

Synonyms

Synonyms Comment Organism
Eco31I
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Escherichia coli