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Literature summary for 3.1.21.4 extracted from
- Kinetic analyses of divalent cation-dependent EcoRV digestions on a DNA-immobilized quartz crystal microbalance (2007), Anal. Biochem., 361, 210-217.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | determination of kinetic parameters for two-step DNA cleavage reactions by the enzyme using a DNA-immobilized 27 Mhz quartz crystal microbalance | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | site-specific binding of enzyme to DNA in presence of Ca2+, without the catalytic cleavage. Binding kinetic parameters in presence of Ca2+ are consistent with those in presence of Mg2+ | Escherichia coli |  |
| Mg2+ | site-specific binding of enzyme to DNA in presence of Mg2+, and site-specific cleavage reaction. Binding kinetic parameters in presence of Ca2+ are consistent with those in presence of Mg2+ | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
isoform EcoRV | - |
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Release 2023.1 (January 2023)
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