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Literature summary for 3.1.21.4 extracted from
- Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence (2005), Structure, 13, 791-801.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure of BstYI bound to a cognate DNA sequence (AGATCT). The structure reveals the basis for degenerate DNA recognition and offers insight into possibilities and limitations in changing the specificities of closely related restriction enzymes | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
recombinant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | BstYI recognizes the degenerate sequence 5'-RGATCY-3' (where R is A/G and Y is C/T) | Geobacillus stearothermophilus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BstYI | - |
Geobacillus stearothermophilus |
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