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Literature summary for 3.1.21.4 extracted from

  • Bellamy, S.R.; Milsom, S.E.; Scott, D.J.; Daniels, L.E.; Wilson, G.G.; Halford, S.E.
    Cleavage of individual DNA strands by the different subunits of the heterodimeric restriction endonuclease BbvCI (2005), J. Mol. Biol., 348, 641-653.
    View publication on PubMed
Search for more literature for 3.1.21.4

Protein Variants

Protein Variants Comment Organism
additional information mutants of BbvCI with defects in one subunit, either R1-R2+ or R1+R2-, cleave only one strand of the 5'-CC-/-TCAGC-3'/5'-GC-/-TGAGG-3' sequence Brevibacillus brevis

Organism

Organism UniProt Comment Textmining
Brevibacillus brevis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O BbvCI cleaves the assymetric DNA sequence, 5'-CC-/-TCAGC-3'/5'-GC-/-TGAGG-3'. The R1 subunit of the enzyme acts at GC-/-TGAGG and the R2 subunit acts at CC-/-TCAGC. the DNA is cleaved initially in one strand, mainly that targeted by the R1 subunit. The other strand is then cleaved slowly by R2 before the enzyme dissociates from the DNA Brevibacillus brevis double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
BbvCI
-
 Brevibacillus brevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
 DNA 37°C, R1+-R2- mutant Brevibacillus brevis
0.0037
-
 DNA 37°C, R1-R2+ mutant Brevibacillus brevis
0.048
-
 DNA 37°C, wild-type enzyme Brevibacillus brevis