General Stability | Organism |
---|---|
in dilute solutions at 37°C, 50% inactivation after 30 min | Lysinibacillus sphaericus |
inactivation by lyophilization | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
high ionic strength | irreversible inactivation | Lysinibacillus sphaericus | |
Zn2+ | 2 mM, complete inhibition | Lysinibacillus sphaericus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lysinibacillus sphaericus | |
Mn2+ | can partially replace Mg2+, optimal concentration is 10 mM | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
enzyme endoR*Bsp | - |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 8-12 months no appreciable loss of activity | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | single strandede DNA and double stranded DNA | Lysinibacillus sphaericus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
endoR*Bsp | - |
Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | some activity is detected even at pH 5 and pH 10 | Lysinibacillus sphaericus |