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Literature summary for 3.1.21.3 extracted from

  • Roberts, G.A.; Houston, P.J.; White, J.H.; Chen, K.; Stephanou, A.S.; Cooper, L.P.; Dryden, D.T.; Lindsay, J.A.
    Impact of target site distribution for Type I restriction enzymes on the evolution of methicillin-resistant Staphylococcus aureus (MRSA) populations (2013), Nucleic Acids Res., 41, 7472-7484.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
duplex DNA + ATP Staphylococcus aureus
-
double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
clones CC1 and CC5
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, DEAE column chromatography, and heparin column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex DNA + ATP
-
Staphylococcus aureus double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate
-
?
additional information does not cut pUC19 plasmid Staphylococcus aureus ?
-
?
plasmid pACYC184 + ATP
-
Staphylococcus aureus ?
-
?
plasmid pET20b + ATP
-
Staphylococcus aureus ?
-
?

Synonyms

Synonyms Comment Organism
EcoAI
-
Staphylococcus aureus
HsdR subunit Staphylococcus aureus
Sau1
-
Staphylococcus aureus
SauMW2I
-
Staphylococcus aureus
SauMW2II
-
Staphylococcus aureus
SauN315I
-
Staphylococcus aureus
SauN315II
-
Staphylococcus aureus
type I restriction enzyme
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Staphylococcus aureus