Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834 (DE3) cells | Vibrio vulnificus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 22% (w/v) polyethylene glycol 8000, 0.02 M imidazole pH 7.5, 5 mM beta-mercaptoethanol | Vibrio vulnificus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, calculated from amino acid sequence | Vibrio vulnificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex DNA + ATP | Vibrio vulnificus | - |
double-stranded DNA fragments with terminal 5'-phosphate + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus | Q7MPU6 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity column chromatography, ion-exchange chromatography, and gel filtration | Vibrio vulnificus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex DNA + ATP | - |
Vibrio vulnificus | double-stranded DNA fragments with terminal 5'-phosphate + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, calculated from amino acid sequence | Vibrio vulnificus |
Synonyms | Comment | Organism |
---|---|---|
HsdM | - |
Vibrio vulnificus |
type I restriction enzyme | - |
Vibrio vulnificus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Vibrio vulnificus |