Cloned (Comment) | Organism |
---|---|
putative HsdM subunit amplified, cloned into pProExHTc, which expresses 25 extra amino acids containing six consecutive His residues at the N-terminus. The expression construct transformed into Escherichia coli B834 (DE3) | Vibrio vulnificus YJ016 |
Crystallization (Comment) | Organism |
---|---|
HsdM subunit, to 1.86 A resolution, by hanging-drop and sitting-drop vapour-diffusion methods. Crystal belongs to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 78.9, c = 165.8 A. With one molecule in the asymmetric unit, the crystal volume per unit protein weight is 2.12 A3/Da, with a solvent content of 42% | Vibrio vulnificus YJ016 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, HsdM subunit, crystallography | Vibrio vulnificus YJ016 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus YJ016 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HsdM subunit, on anion-exchange column, more than 95% pure | Vibrio vulnificus YJ016 |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, HsdM subunit, crystallography | Vibrio vulnificus YJ016 |
Synonyms | Comment | Organism |
---|---|---|
type I restriction enzyme | - |
Vibrio vulnificus YJ016 |