Literature summary for 3.1.21.3 extracted from

Neaves, K.J.; Cooper, L.P.; White, J.H.; Carnally, S.M.; Dryden, D.T.; Edwardson, J.M.; Henderson, R.M.
Atomic force microscopy of the EcoKI type I DNA restriction enzyme bound to DNA shows enzyme dimerization and DNA looping (2009), Nucleic Acids Res., 37, 2053-2063.

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Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
plasmid DNA + H2O	EcoKI prefers to have a partially filled DNA-binding site rather than one fully occupied by non-specific DNA. Dimerization of EcoKI does not occur before DNA binding and takes place on specific sites before any looping. Dimerization occurs before the two specific sites are bought together. Looping initially occurs between a target site and a non-specific region of DNA	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
EcoKI type I DNA restriction enzyme	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Escherichia coli

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Release 2023.1 (January 2023)