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Literature summary for 3.1.21.3 extracted from

  • Mruk, I.; Blumenthal, R.M.
    Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system (2009), Nucleic Acids Res., 37, 983-998.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.3

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplified gene for C.PvuII cloned downstream of the arabinose-inducible PBAD promoter in vector pBAD24 yielding plasmids pIM1 Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli K-12 C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Escherichia coli K-12 ?
-
 ?
additional information C.PvuII binding to operator left activates transcription. Binding preference for operator left over operator right. All four bases of the inter-operator TGTA spacer are required for C.PvuII-dependent activation Escherichia coli K-12 ?
-
 ?

Synonyms

Synonyms Comment Organism
C.PvuII
-
 Escherichia coli K-12
type II restriction-modification system
-
 Escherichia coli K-12