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Literature summary for 3.1.21.3 extracted from
- The BsaHI restriction-modification system: cloning, sequencing and analysis of conserved motifs (2008), BMC Mol. Biol., 9, 48.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged gene cloned into the NheI/EcoRI sites of the pTXBI vector and expressed in Escherichia coli ER2566 | Geobacillus stearothermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D354A | activity is significantly impaired | Geobacillus stearothermophilus |
| E350A | activity is significantly impaired | Geobacillus stearothermophilus |
| F353A | activity is significantly impaired | Geobacillus stearothermophilus |
| P349A | activity is significantly impaired | Geobacillus stearothermophilus |
| Q344A | similar activity to the wild-type | Geobacillus stearothermophilus |
| R351A | activity is negligible | Geobacillus stearothermophilus |
| R352A | similar activity to the wild-type | Geobacillus stearothermophilus |
| S348A | similar activity to the wild-type | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | B0LX59 | - |
- |
| Geobacillus stearothermophilus CPW11 | B0LX59 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Geobacillus stearothermophilus | ? | - |
? |  |
| DNA + H2O | - |
Geobacillus stearothermophilus CPW11 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BsaHI restriction-modification system | - |
Geobacillus stearothermophilus |
| R. BsaHI | - |
Geobacillus stearothermophilus |
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