Literature summary for 3.1.21.3 extracted from

Weiserova, M.; Janscak, P.; Benada, O.; Hubacek, J.; Zinkevich, V.E.; Glover, S.W.; Firman, K.
Cloning, production and characterisation of wild type and mutant forms of the R*EcoK endonucleases (1993), Nucleic Acids Res., 21, 373-379.

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Cloned(Commentary)

Cloned (Comment)	Organism
enzyme with and without a temperature sensitive mutation in the hsdS gene are cloned in pBR322 plamid and introduced into Escherichia coli C3-6	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	enzyme REcoK	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
duplex DNA + ATP	the wild type enzyme EcoK cleaves circular DNA. Only one endonuclease molecule is required per cleavage event. Cleavage of linear DNA may require a second endonuclease molecule	Escherichia coli	double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate	-	?

Subunits

Subunits	Comment	Organism
More	the HsdR subunit may be limiting within the cell. An excess of HsdM and HsdS may produce the methylase in vivo and the assembly of the endonuclease may be dependent upon the prior production of this methylase	Escherichia coli

Synonyms

Synonyms	Comment	Organism
RecoK	-	Escherichia coli

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Release 2023.1 (January 2023)