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Literature summary for 3.1.21.2 extracted from

  • Haas, B.; Sandigursky, M.; Tainer, J.; Franklin, W.; Cunningham, R.
    Purification and characterization of Thermotoga maritima endonuclease IV, a thermostable apurinic/apyrimidinic endonuclease and 3-repair diesterase (1999), J. Bacteriol., 181, 2834-2839 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32400
-
 calculated from sequence Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYJ7
-
-
Thermotoga maritima DSM 3109 Q9WYJ7
-
-

Purification (Commentary)

Purification (Comment) Organism
the gene is expressed under the direction of an IPTG-inducible T7 promoter, and the Thermotoga maritima endonuclease IV is purified as a His-tagged protein Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasmid containing AP sites + H2O the enzyme has apurinic/apyrimidinic endonuclease activity, 3'-phosphatase activity, and 3'-repair diesterase activity for phosphoglycolates and trans-4-hydroxy-2-pentenal-5-phosphates Thermotoga maritima ?
-
 ?
plasmid containing AP sites + H2O the enzyme has apurinic/apyrimidinic endonuclease activity, 3'-phosphatase activity, and 3'-repair diesterase activity for phosphoglycolates and trans-4-hydroxy-2-pentenal-5-phosphates Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32400, calculated from sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
endonuclease IV
-
 Thermotoga maritima
Nfo
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
88
-
 denatures above Thermotoga maritima

General Information

General Information Comment Organism
metabolism the presence of this enzyme, which is part of the DNA base excision repair pathway, suggests that thermophiles use a mechanism similar to that used by mesophiles to deal with the large number of abasic sites that arise in their chromosomes due to the increased rates of DNA damage at elevated temperatures Thermotoga maritima