Literature summary for 3.1.21.2 extracted from

Tsutakawa, S.E.; Shin, D.S.; Mol, C.D.; Izumi, T.; Arvai, A.S.; Mantha, A.K.; Szczesny, B.; Ivanov, I.N.; Hosfield, D.J.; Maiti, B.; Pique, M.E.; Frankel, K.A.; Hitomi, K.; Cunningham, R.P.; Mitra, S.; Tainer, J.A.
Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes (2013), J. Biol. Chem., 288, 8445-8455.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 9.0, 4 mM dithiothreitol, 1.5% saturated MgSO4, 16% (w/v) ethylene glycol, 20% (w/v) polyethylene glycol monomethyl ether 2000	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	contains Mg2+	Thermotoga maritima
Mn2+	contains Mn2+, the enzyme with Mn2+ and Zn2+ is more active than with only Zn2+	Thermotoga maritima
Zn2+	contains Zn2+	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WYJ7	-	-
Thermotoga maritima DSM 3109	Q9WYJ7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
endonuclease IV	-	Thermotoga maritima
Nfo	-	Thermotoga maritima

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Release 2023.1 (January 2023)