Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 130% activity at 1% at pH 8.0 and 37°C | Escherichia coli | |
additional information | the enzyme is induced by phage T4, no activation by S-adenosyl-L-methionine, ATP, or both | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of enzyme activity in naturally occurring mutants of Escherichia coli compared to wild-type strain enzymes, overview | Escherichia coli |
General Stability | Organism |
---|---|
glycerol and bovine serum albumin stabilize | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Chloromercuriphenylsulfonate | - |
Escherichia coli | |
8-hydroxyquinoline | inhibits cleavage of alkylated dsDNA | Escherichia coli | |
Co2+ | - |
Escherichia coli | |
diethyldithiocarbamic acid | - |
Escherichia coli | |
EDTA | inhibits cleavage of non-alkylated dsDNA, 70% inhibition at 0.1 mM | Escherichia coli | |
KCl | 24% inhibition at 0.1 M, 42% at 0.2 M, and 64% at 0.3 M | Escherichia coli | |
additional information | enzyme inhibition by chelating and sulfhydryl agents, but not by tRNA and caffeine, DTT or 2-mercaptoethanol protect against inhibition by sulfhydryl reagents | Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Escherichia coli | |
Mn2+ | stimulates | Escherichia coli | |
additional information | the enzyme shows no absolute requirement for divalent cations, no effect by Ca2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
sucrose density gradient sedimentation equilibrium analysis | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded alkylated DNA + H2O | Escherichia coli | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides | 5'-phosphooligonucleotides | - |
ir | |
double-stranded nativeDNA + H2O | Escherichia coli | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides | 5'-phosphooligonucleotides | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
several strains | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products | reaction mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity in several Escherichia coli wild-type and mutant strains, overview | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 40 mM potassium phosphate, pH 6.5, or 50 mM Tris-HCl, pH 8.0, with 0.1 mM DTT or 2-mercaptoethanol, moderately stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded alkylated DNA + H2O | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides | Escherichia coli | 5'-phosphooligonucleotides | - |
ir | |
double-stranded alkylated DNA + H2O | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, hydrolysis of a phosphodiester bond near an alkylated base in native DNA without single breaks in the region, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 | Escherichia coli | 5'-phosphooligonucleotides | - |
ir | |
double-stranded native DNA + H2O | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 | Escherichia coli | 5'-phosphooligonucleotides | - |
ir | |
double-stranded nativeDNA + H2O | endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides | Escherichia coli | 5'-phosphooligonucleotides | - |
ir | |
additional information | DNA substrate specificity, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease II | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
loss of 41% activity after 30 min, loss of 92% activity after 2 h, with addition 50% glycerol only 6% activity is lost within 2 h | Escherichia coli |
45 | - |
10 min, fraction IV, loss of 10% activity | Escherichia coli |
60 | - |
10 min, fraction IV, complete inactivation | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Escherichia coli |