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Literature summary for 3.1.21.10 extracted from

  • Zhou, R.; Yang, O.; Declais, A.C.; Jin, H.; Gwon, G.H.; Freeman, A.D.J.; Cho, Y.; Lilley, D.M.J.; Ha, T.
    Junction resolving enzymes use multivalency to keep the Holliday junction dynamic (2019), Nat. Chem. Biol., 15, 269-275 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.10

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme cleaves surface-immobilized Holliday junctions in presence of Mg2+ but not in Ca2+. In the presence of Mg2+, Holliday junctions fold into two alternatively stacked conformers. A single Holliday junction can undergo conformer exchange between U1 and U2 and the exchange rate decreases with increasing Mg2+ concentration Escherichia phage T7

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia phage T7 P00641
-
-
Homo sapiens Q96NY9
-
-
Thermochaetoides thermophila G0RYN2
-
-
Thermochaetoides thermophila DSM 1495 G0RYN2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O RuvC binding induces a twofold symmetrical X-shaped Holliday junction structure with two alternative conformers. The enzyme uses multivalency to keep the Holliday junction dynamic Escherichia coli ?
-
 ?
DNA + H2O the enzyme uses multivalency to keep the Holliday junction dynamic Thermochaetoides thermophila ?
-
 ?
DNA + H2O the enzyme uses multivalency to keep the Holliday junction dynamic Escherichia phage T7 ?
-
 ?
DNA + H2O the enzyme uses multivalency to keep the Holliday junction dynamic Homo sapiens ?
-
 ?
DNA + H2O the enzyme uses multivalency to keep the Holliday junction dynamic Thermochaetoides thermophila DSM 1495 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Escherichia coli
dimer
-
 Thermochaetoides thermophila
dimer
-
 Escherichia phage T7
heterodimer hMus81-Eme1 Homo sapiens

Synonyms

Synonyms Comment Organism
Endo I
-
 Escherichia phage T7
endonuclease I
-
 Escherichia phage T7
GEN1
-
 Thermochaetoides thermophila
hMus81
-
 Homo sapiens
RuvC
-
 Escherichia coli