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Literature summary for 3.1.21.10 extracted from

  • Amangyeld, T.; Shin, Y.K.; Lee, M.; Kwon, B.; Seo, Y.S.
    Human MUS81-EME2 can cleave a variety of DNA structures including intact Holliday junction and nicked duplex (2014), Nucleic Acids Res., 42, 5846-5862.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Codon plus (DE3) RIL cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000052
-
 intact Holliday junction in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens
0.00000102
-
 3'-flapped junction in DNA in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ low stimulation of activity at 0.25 mM Homo sapiens
Mg2+ optimum concentration bewtween 0.5 and 2 mM Homo sapiens
Mn2+ optimum concentration bewtween 0.25 and 0.5 mM Homo sapiens
additional information not activated by Ca2+ and Zn2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Homo sapiens the enzyme cleaves a variety of DNA structures including intact Holliday junction and nicked and gapped duplex DNAs generating double-strand breaks. MUS81-EME2 cleaves two strands among three strands present in an nicked duplex. MUS81-EME2 preferentially cleaves nicked duplexes lacking a 5'-phosphate at the nick hydrolyzed DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography and heparin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-flapped junction in DNA + H2O
-
 Homo sapiens ?
-
 ?
DNA + H2O the enzyme cleaves a variety of DNA structures including intact Holliday junction and nicked and gapped duplex DNAs generating double-strand breaks. MUS81-EME2 cleaves two strands among three strands present in an nicked duplex. MUS81-EME2 preferentially cleaves nicked duplexes lacking a 5'-phosphate at the nick Homo sapiens hydrolyzed DNA
-
 ?
intact Holliday junction + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
HJ resolvase
-
 Homo sapiens
Holliday junction resolvase
-
 Homo sapiens
MUS81-EME2
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 37 MUS81-EME2 is more stable at 30°C than 37°C, but remains active at 37°C up to 30 min of incubation Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
 intact Holliday junction in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens
0.354
-
 3'-flapped junction in DNA in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
71830
-
 intact Holliday junction in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens
346300
-
 3'-flapped junction in DNA in 25 mM Tris-HCl, pH 8.0, 1 mM MgCl2, 0.2 mM dithiothreitol, at 37°C Homo sapiens