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Literature summary for 3.1.21.10 extracted from
- Crystal structure of RuvC resolvase in complex with Holliday junction substrate (2013), Nucleic Acids Res., 41, 9945-9955.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with a synthetic Holliday junction, using 0.1 M citric acid (pH 3.5) and 1.5 M NaCl in 0.3-0.5M ammonium phosphate | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E70Q | inactive | Thermus thermophilus |
| H143D | the mutant shows increased activity compared to the wild type enzyme | Thermus thermophilus |
| L80C | inactive | Thermus thermophilus |
| M108C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
| P40C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
| Q77C | inactive | Thermus thermophilus |
| R140C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
| R76C | inactive | Thermus thermophilus |
| T11C | the mutant shows reduced activity compared to the wild type enzyme | Thermus thermophilus |
| Y75A | the mutant shows markedly increased activity compared to the wild type | Thermus thermophilus |
| Y75A/H143D | the mutant shows increased activity compared to the wild type enzyme | Thermus thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | requires divalent metal ions for activity | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Thermus thermophilus | - |
hydrolyzed DNA | - |
? |  |
| DNA + H2O | Thermus thermophilus DSM 579 | - |
hydrolyzed DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q5SJC4 | - |
- |
| Thermus thermophilus DSM 579 | Q5SJC4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel affinity column chromatography and Mono-S column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Thermus thermophilus | hydrolyzed DNA | - |
? | |
| DNA + H2O | - |
Thermus thermophilus DSM 579 | hydrolyzed DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | X-ray crastallography | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Holliday junction resolvase | - |
Thermus thermophilus |
| RuvC | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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