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Literature summary for 3.1.21.10 extracted from
- Structural asymmetry in the Thermus thermophilus RuvC dimer suggests a basis for sequential strand cleavages during Holliday junction resolution (2013), Nucleic Acids Res., 41, 648-656.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 35% (w/v) polyethylene glycol 3350, 0.2M Li2SO4 and 0.1M Tris-HCl (pH 8.5) | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D146N | inactive | Thermus thermophilus |
| F73A | the mutant shows 50% reduced activity compared to the wild type enzyme | Thermus thermophilus |
| F74A | the mutant shows 30% reduced activity compared to the wild type enzyme | Thermus thermophilus |
| Y75A | the mutant shows about 1.9fold increase of activity compared to the wild type enzyme | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Thermus thermophilus | - |
hydrolyzed DNA | - |
? |  |
| DNA + H2O | Thermus thermophilus DSM 579 | - |
hydrolyzed DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q5SJC4 | - |
- |
| Thermus thermophilus DSM 579 | Q5SJC4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Hi-trap heparin-Sepharose column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | - |
Thermus thermophilus | hydrolyzed DNA | - |
? | |
| DNA + H2O | - |
Thermus thermophilus DSM 579 | hydrolyzed DNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HJ resolvase | - |
Thermus thermophilus |
| Holliday junction resolvase | - |
Thermus thermophilus |
| RuvC | - |
Thermus thermophilus |
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Release 2023.1 (January 2023)
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