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Literature summary for 3.1.21.10 extracted from

  • Rass, U.
    Resolving branched DNA intermediates with structure-specific nucleases during replication in eukaryotes (2013), Chromosoma, 122, 499-515.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.10

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Homo sapiens the substrate spectrum of MUS81-EME1 comprises 3'-flaps (duplex DNA with a 3'-single-stranded flap), double-stranded three-way junctions that resemble replication forks, Holliday junction precursors, and fully ligated Holliday junctions. Slx1–Slx4 cleaves splayed arm DNA substrates (a duplex with unpaired 3'- and 5'-overhangs on one side), 5'-flaps (duplex DNA with a 5'-single-stranded flap), replication forks, and Holliday junctionss hydrolyzed DNA
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Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Homo sapiens
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-
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Saccharomyces cerevisiae
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the substrate spectrum of MUS81-EME1 comprises 3'-flaps (duplex DNA with a 3'-single-stranded flap), double-stranded three-way junctions that resemble replication forks, Holliday junction precursors, and fully ligated Holliday junctions. Slx1–Slx4 cleaves splayed arm DNA substrates (a duplex with unpaired 3'- and 5'-overhangs on one side), 5'-flaps (duplex DNA with a 5'-single-stranded flap), replication forks, and Holliday junctionss Homo sapiens hydrolyzed DNA
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 ?

Subunits

Subunits Comment Organism
monomer GEN1 Homo sapiens

Synonyms

Synonyms Comment Organism
Eme1
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 Homo sapiens
GEN1
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 Homo sapiens
HJ-resolving nuclease
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 Escherichia coli
HJ-resolving nuclease
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 Homo sapiens
HJ-resolving nuclease
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 Saccharomyces cerevisiae
Holliday junction resolvase
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 Escherichia coli
Holliday junction resolvase
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 Homo sapiens
Holliday junction resolvase
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 Saccharomyces cerevisiae
Mus81
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 Homo sapiens
Mus81
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 Saccharomyces cerevisiae
RuvC
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 Escherichia coli
Slx1
-
 Homo sapiens
Slx4
-
 Homo sapiens
Yen1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction loss of Mus81 activity sensitizes yeast cells to a large panel of agents whose effects inhibit RF progression, including methyl methanesulfonate, UV light, camptothecin, hydroxyurea, and DNA cross-linking agents Saccharomyces cerevisiae
physiological function RuvC cleaves Holliday junctions by the introduction of two symmetrically related nicks. The enzyme also cuts branched DNA intermediates that originate directly from blocked replication forks, targeting them for origin-independent replication restart Escherichia coli