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Literature summary for 3.1.21.10 extracted from
- Two Holliday junction resolving enzymes in Sulfolobus solfataricus (2000), J. Mol. Biol., 297, 923-932.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strongly dependent on the presence of magnesium, with 15 mM Mg2+ ions optimal. Strong influence of magnesium ion concentrations on protein-junction complex stability. A dramatic shift in binding affinity is observed, with apparent dissociation constants for binding in the presence of 1.5, 2.5, 5 and 15 mM magnesium ions of 76 nM, 530 nM, 1100 nM and 1700 nM, respectively | Saccharolobus solfataricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16010 | - |
x * 16010, calculated from sequence | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q7LXU0 | - |
- |
| Saccharolobus solfataricus P2 | Q7LXU0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Holliday junction in DNA + H2O | Sulfolobus has two distinct junction resolving enzymes, Hjc and Hje, with differing substrate specificities. The Hje and Hjc activities display pronounced differences in their patterns of cleavage of the junction, and no nucleotide positions are cleaved by both enzymes. Whilst Hje introduces multiple nicks in each arm of the mobile junction Jbm5, Hjc cuts each arm at only a single site. Hje introduces paired nicks on only the continuous h and x strands of the fixed junction J3, two nucleotides 3' of the point of strand exchange. In contrast, recombinant Hjc cleaves all four strands of the junction, with cleavage three nucleotides 3' of the junction centre | Saccharolobus solfataricus | ? | - |
? | |
| Holliday junction in DNA + H2O | Sulfolobus has two distinct junction resolving enzymes, Hjc and Hje, with differing substrate specificities. The Hje and Hjc activities display pronounced differences in their patterns of cleavage of the junction, and no nucleotide positions are cleaved by both enzymes. Whilst Hje introduces multiple nicks in each arm of the mobile junction Jbm5, Hjc cuts each arm at only a single site. Hje introduces paired nicks on only the continuous h and x strands of the fixed junction J3, two nucleotides 3' of the point of strand exchange. In contrast, recombinant Hjc cleaves all four strands of the junction, with cleavage three nucleotides 3' of the junction centre | Saccharolobus solfataricus P2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16010, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hjc protein | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharolobus solfataricus |
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