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Literature summary for 3.1.21.10 extracted from
- Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2 (2011), PLoS ONE, 6, e23668.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning in Escherichia coli strain NEB 5 alpha, and expression in strain NEB Turbo and NEB T7 Express | Sulfolobus islandicus rod-shaped virus 2 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Sulfolobus islandicus rod-shaped virus 2 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sulfolobus islandicus rod-shaped virus 2 | enzyme substrate specificity, overview. A specific interaction occurs between SIRV2 Holliday junction resolving enzyme, Hjr, and the SIRV2 virion body coat protein, SIRV2gp26 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus islandicus rod-shaped virus 2 | - |
SIRV2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme substrate specificity, overview. A specific interaction occurs between SIRV2 Holliday junction resolving enzyme, Hjr, and the SIRV2 virion body coat protein, SIRV2gp26 | Sulfolobus islandicus rod-shaped virus 2 | ? | - |
? | |
| additional information | usage of two plasmids containing hairpin four-way junctions constructed to assay resolving enzyme activity | Sulfolobus islandicus rod-shaped virus 2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Hjr | - |
Sulfolobus islandicus rod-shaped virus 2 |
| Holliday junction resolving enzyme | - |
Sulfolobus islandicus rod-shaped virus 2 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Sulfolobus islandicus rod-shaped virus 2 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
assay at | Sulfolobus islandicus rod-shaped virus 2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Holliday junction resolving enzyme is a key enzyme in SIRV2 genome replication. Modeling linking SIRV2 Holliday junction resolving enzyme genome resolution to viral particle assembly, overview | Sulfolobus islandicus rod-shaped virus 2 |
| physiological function | molecular mechanism for the Holliday junction resolving enzyme four-way junction cleavage bias, minimal requirements for four-way junction cleavage, and substrate specificity. The cleavage is specific to four-way DNA junctions and inactive on other branched DNA molecules | Sulfolobus islandicus rod-shaped virus 2 |
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Release 2023.1 (January 2023)
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