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Literature summary for 3.1.21.10 extracted from

  • Canas, C.; Carrasco, B.; Garcia-Tirado, E.; Rafferty, J.B.; Alonso, J.C.; Ayora, S.
    The stalk region of the RecU resolvase is essential for Holliday junction recognition and distortion (2011), J. Mol. Biol., 410, 39-49.
    View publication on PubMed
Search for more literature for 3.1.21.10

Protein Variants

Protein Variants Comment Organism
F81A naturally occuring mutant, that is sensitive to DNA-damaging agents as a null recU strain, a severely impaired enzyme. The mutant poorly recognizes and distorts Holliday junctions. At high concentrations, RecUF81A binds to Holliday junctions but fails to cleave them. RecUF81A does not inhibit RecA dATPase and strand-exchange activities and it loses structural selectivity for X-shaped structures. Phenotype, detailed overview Bacillus subtilis
Y80A naturally occuring mutant showing an an intermediate phenotype. The mutant poorly recognizes and distorts Holliday junctions, and cleaves them with low efficiency. RecUY80A does not inhibit RecA dATPase and strand-exchange activities and it loses structural selectivity for X-shaped structures. Phenotype, detailed overview Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis RecU has two activities: in concert with RuvAB, it catalyzes the resolution of Holliday junctions, and, alone, it modulates RecA activities. RecU does not modulate RecA when it is bound to the Holliday junction ?
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 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information RecU has two activities: in concert with RuvAB, it catalyzes the resolution of Holliday junctions, and, alone, it modulates RecA activities. RecU does not modulate RecA when it is bound to the Holliday junction Bacillus subtilis ?
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 ?

Subunits

Subunits Comment Organism
More the RecU structure shows a mushroom-like appearance, with a cap and a stalk region. The RuvB interaction and the catalytic residues are located in the cap region of dimeric RecU, while the stalk region is essential not only for RecA modulation but also for Holliday junction recognition Bacillus subtilis

Synonyms

Synonyms Comment Organism
RecU resolvase
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 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Bacillus subtilis

General Information

General Information Comment Organism
physiological function the RecU protein has two activities: to recognize, distort, and cleave four-stranded recombination intermediates and to modulate RecA activities. The RuvB interaction and the catalytic residues are located in the cap region of dimeric RecU, while the stalk region is essential not only for RecA modulation but also for Holliday junction recognition Bacillus subtilis