Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity with different constructs of Y-DNA under various packaging conditions, T4 endo VII resolvase cleavage sites in Y-DNAs, overview | Tequatrovirus T4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease VII resolvase | - |
Tequatrovirus T4 |
T4 endo VII resolvase | - |
Tequatrovirus T4 |
T4 gp49 endonuclease VII resolvase | - |
Tequatrovirus T4 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Tequatrovirus T4 |
General Information | Comment | Organism |
---|---|---|
physiological function | in vivo the T4 phage packaging motor deals with Y- or X-structures in the replicative concatemer substrate by employing a portal-bound Holliday junction resolvase that trims and releases these DNA roadblocks to packaging. Purified T4 gp49 endonuclease VII resolvase can release DNA compression in vitro in prohead portal packaging motor anchored and arrested Y-DNA substrates. Conformational changes in both the motor proteins and the DNA substrate itself that are associated with the power stroke of the motor are consistent with a proposed linear motor employing a terminal-to-portal DNA grip-and-release mechanism | Tequatrovirus T4 |