Any feedback?
Literature summary for 3.1.21.10 extracted from
- ruvA Mutants that resolve Holliday junctions but do not reverse replication forks (2008), PLoS Genet., 4, e1000012.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E68G/H136R | the mutation affects octamer formation, DNA binding, and the stimulation of RuvB helicase activity | Escherichia coli |
| H29R/E40G/E68G/K129E/F140S/S177G/D184N | the mutation affects octamer formation, DNA binding and the stimulation of RuvB helicase activity | Escherichia coli |
| H29R/E40G/Q58R/K129E/F140S/S177G/D184N | the mutation affects octamer formation, DNA binding and the stimulation of RuvB helicase activity | Escherichia coli |
| N79D/N100D | the mutation affects octamer formation, DNA binding and the stimulation of RuvB helicase activity | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | RuvA also stimulates RuvB helicase activity | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Holliday junctions in DNA + H2O | - |
Escherichia coli | ? | - |
? | |
| additional information | RuvA also stimulates RuvB helicase activity | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | active enzyme, in the presence of Mg2+, SDS-PAGE | Escherichia coli |
| tetramer | SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HJ resolvase | - |
Escherichia coli |
| Holliday junction resolvase | - |
Escherichia coli |
| RusA | - |
Escherichia coli |
| RuvABC complex | resolves Holliday junctions to produce recombinant molecules | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
